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Recently, interesting reports on the relation between cAMP levels of the cells and the immune response have been presented. This time the author studied on the cAMP levels of lymphocytes (lymphnode, spleen and thymus) in the guinea pig and the effect of the addition of isopreterenol and theophyllin.<BR>The lymphocyte suspension was prepared from lymphnode, spleen and thymus of the guinea pig and the distribution percent of T-cells and B-cells was counted with the technique of RRBC (rabbit red blood cell)-rosette formation and EAC-rosette formation. T-cells were isolated from lymphnode cell suspension passing through glass wool column and they were further purified with twice passage through nylon wool column.<BR>The summarized results indicated that cAMP levels in lymphnode, spleen and thymus cell suspension generaly increased about 135-230 percent by the addition of isopreterenol, theophyllin and the combination of both compared to control, but any increase of cAMP level was not observed in the case of purified T-cells of lymphnode origin.
- 日本医科大学医学会の論文
日本医科大学医学会 | 論文
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