Grouping of Tumorigenic Agrobacterium spp. Based on Ti Plasmid-Related Phenotypes.
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概要
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Nineteen strains of Agrobacterium tumefaciens and 2 strains of A. rubi were tested for host range (stems of grapevine, peach, pear, tomato, sunflower, Kalanchoe daigremontiana and Nicotiana tabacum, and leaves of K. daigremontiana), tumor morphology on K. daigremontiana and grapevine, opine induction in tomato tumors, and agrocin 84 sensitivity. By principal component analysis using the mean diameter of 60 tumors formed by each strain on each of the test plants as a variable, the total of 21 strains were separated into the following seven groups named Groups 1-7. Group 1 (octopine and cucumopineinducing strains), Group 2 (vitopine-inducing strains) and Group 3 (opine class was not determined) consisted of only biovar 3 strains, showed strong pathogenicity to grapevine, but differed in pathogenicity to other plants. Group 4 (octopine, agropine and mannopine-inducing strains) consisted of only biovar 1, whereas Group 5 (nopaline-inducing strains) comprised biovars 1 and 2, A. rubi and unclassified strains isolated from cherry, indicating its heterogeneity in chromosomal markers. Groups 4 and 5 showed weak pathogenicity to grapevine, but strong pathogenicity to the other plants. Both of Group 6 (opine class was not determined) and Group 7 (nopaline-inducing strains) contained unclassified strains (NCPPB 1650 and two kiwifruit isolates, respectively), and showed weak pathogenicity to all the inoculated plants. Strains belonging to each group gave uniform reactions within the group in tumor morphology, opine induction and agrocin 84 sensitivity, except for Group 5 which was divided into 2 subgroups (A and B) based on agrocin 84 sensitivity. Agrocin 84-sensitive Group 5-A consisted of biovars 1, 2 and unclassified cherry isolates, and resistant Group 5-B comprised only A. rubi. All the strains except for Group 5-A were agrocin-84 resistant. Six Japanese strains of biovar 3 belonging to Group 2 induced vitopine, indicating that the vitopine strains have a wide distribution in Japan.
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