Characteristics of epitopes on the tomato strain of tobacco mosaic virus detected by monoclonal antibodies.
スポンサーリンク
概要
- 論文の詳細を見る
Eleven murine monoclonal antibodies (MABs) specific for L11A, a tomato strain of tobacco mosaic virus (TMV-L11A), were produced in culture supernatants and in ascitic fluids. Reactivities of all the 11MABs with tobamovirus strains were investigated by two indirect ELISA procedures, competitive ELISA, precipitin ring interface test and immuno-double-diffusion test. Five MABs among them reacted only with tomato strains (L11A, L and CH2) and the others reacted not only with tomato strains but also with other strains [TMV-OM, P, W and cucumber strain of cucumber green mottle mosaic virus (CGMMV-Cu)]. Based on these results, at least eight epitopes were distinguished on the surface of the particles of L11A. The epitopes, except for one detected by one of the MABs (6F11), were all neotopes, and 10 out of 11MABs were anti-neotope antibodies including a heterospecific antibody (8C8). A superior epitope which reacted mainly in the antiserum was identified within these epitopes. The high specificity of the MABs showed that they were useful for the differentiation of tobamovirus strains. The titers of three MABs in ascitic fluids were 1:2, 560 and they could be substituted for rabbit antisera. This study showed that the antigenic specificity of the strains was mainly dependent on the neotopes.
- 日本植物病理学会の論文
日本植物病理学会 | 論文
- 育苗圃におけるイチゴ炭疽病の潜在感染率の推移と発病との関係(関東部会講演要旨,平成18年度地域部会講演要旨)
- (171)北海道の田畑輪換圃場を利用したイチゴリレー苗生産におけるColletotrichum gloeosporioidesの潜在感染とその病原性(平成14年度 日本植物病理学会大会講演要旨)
- (170)北海道の田畑輪換圃場を利用したイチゴリレー苗生産による炭疽病・萎凋病の防除(平成14年度 日本植物病理学会大会講演要旨)
- (47) Alternaria alternataの形質転換体における外来遺伝子の増幅 (平成2年度大会講演要旨)
- (8) 相同的組み換えによるAlternaria alternataの形質転換と形質転換体における外来遺伝子の安定性 (関西部会講演要旨)