Production of monoclonal antibodies against rice stripe virus for the detection of virus antigen in infected plants and viruliferous insects.

概要

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Hybridoma cells that secrete monoclonal antibodies against rice stripe virus (RSV) were produced by fusing spleen cells of a BALB/c mouse immunized with RSV to a mouse myeloma cell line P3-X63-Ag8-U1 (P3U1). A single stable cell line with the highest titer in the avidin-biotin-peroxidase system to the homologous antigen was screened. The titer of the hybridoma ascitic fluid increased with the number of days after the injection of hybridoma cells to mice. The titers of the ascitic fluid were 800-1, 600 in precipitin ring interface test. The yield of the ascitic fluid was 11.4-22.5ml for a single mouse and was ca. 100ml for 6 mice. The titer and the yield of the ascitic fluid decreased with the serial passage of the hybridoma to new mice and the titers became about half that of the initial value after one to two passages through mice. The monoclonal antibodies were successfully used to detect virus antigen in virus infected plants and viruliferous insects by the latex flocculation test and enzyme-linked immunosorbent assay.
日本植物病理学会の論文