Production of Oospore-Encrusted Seeds by Inoculation of Conidia of Downy Mildew Fungus, Peronospora manshurica, to Soybean Flower and Pod

概要

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Conidium suspension of soybean downy mildew fungus, Peronospora manshurica, was inoculated to floral organ of 5 soybean cultivars 30-33days after sowing and to young pod after 40-43days. After harvesting, the percentage of oospore-encrusted seeds was estimated. Oospore-encrusted seeds were found to be formed after either flower or pod inoculation, and the percentage of oospore-encrusted seeds was high in Denko-okuharawase and Okuharawase cultivars and low in Hakucho, Raicho and Wasemidori cultivars. On the innermost layer of pod in contact with ospore-encrusted seed, mass of oospores was also found. As a result of a survey involving 38 samples from 27 cultivars collected from various parts of Japan, the percentage of oospore-encrusted seeds was found to range between 0.1 and 23.4% in 34 samples from 23 cultivars, while oospore-encrusted seeds were not observed in 4 samples from 4 cultivars. Difference in percentage related to the localities where sample collection was made could not be detected.
日本植物病理学会の論文