Is It Insulin That is Stained by Aldehyde Fuchsin and Pseudoisocyanin in the Sections of the Pancreas?
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Rabbits were intravenously given alloxan (200mg/kg) and the changes in the cytology of the pancreatic B cells as well as in the insulin content of the pancreas were examined. Although 26% of insulin was shown to have been lost from the pancreas in 30min and 41% in 24hrs after the administration of alloxan, no decrease was found in the amount of the substance stained by aldehyde fuchsin and reacting metachromatically to pseudoisocyanin. The content of zinc in the B cells examined by the dithizon method diminished considerably in 30min and almost disappeared in the 24hr case. These results indicate that it is not, or at least not only, insulin that is responsible for the aldehyde fuchsin and pseudoisocyanin stainings.On the other hand, it was confirmed, that the stained substance in the B cells decreased in amount in clear concurrence with the insulin content of the pancreas when the guinea pig was given an intraperitoneal injection of glucose (2g/kg). This implies that the substance reacting in both stainings corresponds to a certain structure which is associated with insulin as far as the cell physiologically functions. A hypothesis that the substance is in the membrane sac surrounding the beta granule seems to fit in with this condition.
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国際組織細胞学会 | 論文
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