Biological Studies on Light Quality in Environment Control:I. Examination of Irradiated Monochromatic Light Purity Using Protochlorophyllide Phototransformation and Chlorophyll Accumulation as the Index
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1. Examination of irradiated monochromatic light purity was attempted using protochlorophyllide phototransformation to chlorophyllide <I>a</I> and chorophyll <I>a</I> accumulation as the index. Examination was carried out in the region from 700 am to 800 nm. Monochromatic light was obtained from a Grating Spectroirradiater, Biological Spectrograph.<BR>2. Twelve % of the phototransformation found by irradiation with 650 nm light was prefered as the most efficient light for phototransformation, at 10 erg/cm<SUP>2</SUP>/sec for 10 min. However, phototransformation was not detected by irradiation above 700 am light at 20, 000 erg/cm<SUP>2</SUP>/sec for 10 min. By simultaneous irradiation with 720 nm and 650 nm light, the same level of phototransformation, in comparison with the value of 650 nm alone, was observed. The extent of stray flux in light above 700 am at 20, 000 erg/cm2/sec might be considered at the level of intensity that has an equivalent relationship concerning phototransformation between stray flux and 650 nm light. Phototransformation is useful as the index of monochromatic light purity.3. By irradiation with monochromatic lights above 700 nm at 20, 000 erg/cm<SUP>2</SUP>/sec for 20 hours, a peak of chlorophyll accumulation was observed in the 720 nm region. From evidence that the induction period of accumulation by irradiation in this region is longer than that for 650 nm light and that the remaining photoconvertible protochlorophyllide was found in greening leaves, then chlorophyll accumulation in the 720 nm region might be due to unknown effects of 720 nm intense light. Chlorophyll accumulation in this region was unsuitable for use as an index in examining stray flux.<BR>4. Using 780 nm light irradiation at 20, 000 erg/cm<SUP>2</SUP>/sec for 20 hours, 3μ<I>g</I>/<I>g</I> F. W. of chlorophyll content was found. No chlorophyll was found in leaves irradiated by 790 nm and 800 nm lights. Therefore, chlorophyll accumulation in the 790-800 nm region was used as the index.
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