Effects of Sucrose and Polyethylene Glycol on Leaf Emergence, Rooting and Bulblet Growth of Fritillaria camtschatcensis Ker-Gawl. In Vitro Culture.

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Experiments were conducted to determine the effects of sucrose and polyethylene glycol (PEG) on leaf emergence, rooting and growth of the regenerated bulblets of Fritillaria camtschatcensis Ker-Gawl. which were formed on the small globule-scale of the bulbs in vitro culture. The bulblets were cultured on MS medium containing sucrose of 0 (Cont.), 30, 60, 90 and 120 g/l or PEG of 0, 10, 30 and 50 g/l for 8 weeks under continuous light of 40 μmol m-2 s-1 at 20°C in vitro. Also NAA of 0.1 mg/l and kinetin of 0.1 mg/l were supplied to the media. Sucrose concentration of 60 g/1 was suitable for leaf emergence and 60 and 120 g/1 were suitable for rooting from the bulblets. And the concentrations of 30 and 60 g/1 were effective to increases in bulblet growth. PEG was effective for rooting but no effect on leaf emergence. The concentration of 10 g/1 was optimum condition to increase in bulblet growth. Summed up these results, sucrose of 30 to 60 g/1 and PEG of 10 g/1 are the most suitable concentration for bulblet growth of Fritillaria camtschatcensis Ker-Gawl.
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