Improvement of Synchronization on Carrot Somatic Embryo Culture by Controlling Dissolved Oxygen Concentration.

概要

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Low synchronization of somatic embryogenesis prevents the application of a large-scale production bioreactor to a transplant production system. We attempted to improve synchronization of carrot somatic embryogenesis for large-scale production by controlling the dissolved oxygen concentration. A suitably low dissolved oxygen concentration repressed excessive development of cotyledonary-stage embryos but did not affect the development of globular, heart, and torpedo-stage embryos. As a result the development of somatic embryos stopped at the torpedo stage under this condition. Moreover, the number of torpedo-stage embryos increased, and it was larger than the maximum number of torpedo-stage embryos in culture with ambient oxygen aeration. These torpedo-stage embryos regenerated normal plantlets after mild dehydration treatment. This method does not require changing the medium nor filtration of the somatic embryos during the culture period for synchronization of the culture. If this simple method is used for large-scale production of somatic embryos with a bioreactor, effective synchronization can be achieved by only controlling the dissolved oxygen concentration.
日本生物環境工学会の論文