Viability of Calli from Hypocotyl of Kiwifruit Seedlings Exposed to Liquid Nitrogen.
スポンサーリンク
概要
- 論文の詳細を見る
Seeds obtained from kiwifruit (<I>Actinidia deliciosa</I> (A. chev.) C. F. Liang <I>et</I> A. R. Ferguson var. <I>deliciosa</I> cv. Hayward) were germinated and the calli derived from the hypocotyl of the seedlings were used to test various methods of cryopreservation. Some calli were found to be preserved after immersing in liquid nitrogen (LN) . The effective measures for preserving calli after immersing in LN are as follows ; calli were cultured with 24% (w/v) or 41% (w/v) sucrose for 2 days, and then after being dehydrated twice, first for 22 min with 60% (v/v) PVS2 (30% (w/v) glycerol, 15% (w/v) dimethyl sulfoxide, 15% (w/v) ethylene glycol and 13.7% (w/v) sucrose) and then for 23 min with 100% (v/v) PVS2, were preserved in LN. After cryopreservation, the calli were warmed in 37°C water immediately after being taken from the LN. They were washed in a liquid culture medium containing highly concentrated sucrose and then revived in a medium from which ammonium nitrate (NH<SUB>4</SUB>NO<SUB>3</SUB>) had been removed. These results will be important to the development of cryopreservation methods of kiwifruit germplasm.
- 日本生物環境工学会の論文
日本生物環境工学会 | 論文
- 温室における燃焼式蓄熱型二酸化炭素施肥システムの省エネルギー性・環境負荷低減性・経済性評価
- 高濃度培養液を用いる高糖度トマト生産に適した循環キャピラリー栽培システムの開発
- 2種のUV励起光によるラッカセイ生葉のLIFスペクトル変化および蛍光葉内分布
- ナメコ子実体の光形態形成過程における発現遺伝子の解析
- 光の波長と環境温度がナメコの生体電位および形態形成に及ぼす影響