The morphological characteristics of interspecific hybrids with Zoysia minima and its application for breeding
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概要
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Ten hybrid plants were obtained by crossing between <I>Zoysia minima</I>, indigenous plant to New Zealand, and 5 other <I>Zoysia</I> species grown in Japan. In this study, the hybridity of the F<SUB>1</SUB> plants were confirmed by RAPD (Random Amplified Polymorphic DNA), and subsequently, the observation of morphological and growth characteristics was conducted. As a result of the RAPD using the selected 31 random primers, the F<SUB>1</SUB> plants obtained were a hybrid between the <I>Z. minima</I> and <I>Zoysia</I> species. Microscopic examination of the transverse section of leaf blade indicates C-B-type streamlining on both sides of the leaf edge in all hybrids with <I>Z. minima</I>. In addition, the pollen fertility of F<SUB>1</SUB> plants was high over 80%, and this pollen was almost normal. In the characteristics observation of hybrids with <I>Z. minima</I> as the mother plant, the widths of leaves were narrow, and the forms of the spikes became small, with a tendency towards a smaller size. <I>Z. minima</I> was an effective germplasm stock for cases in which the breeding is advanced in the direction that miniaturizes plant type by interspecific hybrid.
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