Transgenic plants of creeping bentgrass(Agrostis palustris Huds.) 'Chiba green B-1' by particle bombardment.
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概要
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The <I>bar</I> gene was introduced into the suspension culture of creeping bentgrass (<I>Agrostis palustris</I> Huds.) 'Chiba green B-1', a "Brown patch" -resitant cultivar, co-transformed with the <I>gus</I> or <I>cry I A</I> (<I>c</I>) FM gene by particle bombardment. 16 transgenic plants were obtained after the selecion on the medium containing bialaplios.<BR>PCR and PCR-Southern hybridization analyses showed that all the bialaphos resistant regenerants tested carried the <I>bar</I> gene . Some plants appeared to be cointegrated with the <I>gus</I> or <I>cry IA</I> (<I>c</I>) FM gene.<BR>Expression of the <I>bar</I> gene was detected by the determination of ammonia content in leaf extract after spraying with 0.5 % (Vol/Vol) HERBIE<SUP>TM</SUP>. Ammonia contents of transgenic plants were very low as compared with untransformed control. Expression of the <I>cry IA</I> (<I>c</I>) FM gene is under investigation.
- 日本芝草学会の論文
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