Nutritional Enrichment and Cultivation of Rotifers by Feeding of Docosahexaenoic Acid-enriched Chlorella vulgaris K-22
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概要
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The cultivation of rotifers using docosahexaenoic acid (DHA) -enriched <I>Chlorella vulgaris</I> K-22 as a food was investigated. The exogenous DHA was incorporated into neutral lipids in DHA enriched <I>C. vulgaris</I> cells. The highest percentage of DHA in the total fatty acids of cellular lipids reached 82.9%. During the nutritional enrichment of rotifers with DHA enriched <I>C. vulgaris, </I> the amount of DHA in the rotifers rapidly increased to 3.3% over 6 hours. During the course of the 3-day cultivation of the rotifers, those provided with DHA-enriched <I>C. vulgaris</I> showed higher population growth, and maintained higher stable levels of DHA, than rotifers that were given non-enriched <I>C. vulgaris.</I> Thus, cultivation by providing rotifers with DHA enriched <I>C. vulgaris</I> resulted in rotifers possessing high DHA content without the use of additional nutritional enrichment procedures.
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