Early Rearing Conditions of Hotoke loach, Lefua echigonia.
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概要
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We have developed technology for producing seeding of the Hotoke loach, <I>Lefua echigonia</I>, designated as an endangered species by the Environment Agency and Kanagawa Prefecture. In selective of spawning bed experiment, Hotoke loaches were matured in a 100<I>l</I> PC tank with increased water temperature and long-day treatment, and natural spawning was induced using several types of aquatic plants. While the highest spawning was achieved with Uiromosu, <I>fantinalis antipyreticus</I>, spawning was also observed with Kinran, an artificial water plant. In experiments of mass egg-taking, designed to increase production, batches of 10 male/female parent fish were released into a 2tFRP tank with circulating filtration, and spawning was induced using Kinran. A total of 2, 179 hatched larvae were obtained from 1-year-old parents and 452 from 2-year-olds.<BR>We conducted rearing trials using the hatched larvae. Hatched larvae, breeding experiments under prefectual administration, we reared six groups of hatched larvae in 56<I>l</I> glass tanks with circulating filtration. Between 60 and 300 larvae were accommodated in each tank and were fed rotifer, <I>Aytemia</I>, and particulate feed. The survival rate between 50 and 100 days was 12.3-77.0%, and seedlings with a body length of 2-3cm were produced. In hatched larvae food experiment, we conducted a study trial on initial feeding in a 36<I>l</I> glass tank. We found that the larvae could be reared with a combination of <I>Artemia</I> and particulate feed, excluding rotifers. In experiments of mass seed production, aimed at increased production, we reared hatched larvae via circulating filtration in three 2tFRP tanks. With a combination of <I>Artemia</I> and particulate feed, approximately 5, 000 seedlings (3cm size) were produced between 121 and 144 days.
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