Monosex Female Production by the Use of Sex-reversed Males of Nigorobuna, Carassius carassius grandoculis.

概要

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Production of all-female progeny in Nigorobuna, <I>Carassius carassius grandoculis</I> was attempted with sex-reversed males. Gynogenetic diploids of nigorobuna were produced by the cold-shock treatment of the eggs for preventing the extrusion of the second polar body. Administration of 17-methyltestosterone for the sex reversal from female to male was performed by dissolution in the rearing water at 1.0μg/l (8h per day) for 60 days from 25 days after hatching. Proportion of males in the hormone-treated fish increased significantly. Since these males produced almost all female offspring, they were regarded as sex-reversed males. Sex-reversed males used at a commercial level allowed the production of all-female progeny. From these results, the sex determining system of Nigorobuna was presumed to be female homogamety.
日本水産増殖学会の論文