Studies on the Conditions for Induction of Mitotic-gynogenetic Deploids in Red Sea Bream, Pagrus major by Hydrostatic Pressure.
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We tried to determine the optimum conditions for induction of mitotic gynogenetic diploids (mitotic-G2n) in red sea bream, <I>Pagrus major</I>. The mitotic-G2n was induced by blocking the first mitotic division using a hydrostatic pressure treatment. We tested several combinations of pressure intensity, duration time, and starting time after insemination and observed hatching-rate, diploid embryo-rate (2n-rate), and haploid embryo-rate (n-rate) in each experimental group. The optimum conditions for mitotic-G2n induction in red sea bream were pressure intensity of 700kg/cm<SUP>2</SUP> for 5 min duration, starting at 45 min after insemination in the incubation temperature. Hatching-rate was 35.06%, diploid embryorate, 53.41% and haploid embryo-rate, 46.59%. Nevertheless, a considerably high number of heteroploids appeared and high mortality was observed at the metamorphosis stage, so further investigation is needed.
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日本水産増殖学会 | 論文
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