Cryopreservation of Conchocelis of Porphyra yezoensis form. narawaensis.

概要

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For a long term storage, conchocelis cells of<I>Porphyra yezoensis</I>form.<I>narawaensis</I> were frozen and stored in liquid nitrogen. Conchocelis cells were frozen by a two-step procedure having an initial slow freezing rate (1°C/min) to -40°C followed by a rapid freezing rate when samples plunged into liquid nitrogen. A cryoprotective solution containing dimethyl sulfoxide and sorbitol was necessary for survival of conchocelis cells during the freeze-thaw cycle. After 12 months storage in liquid nitrogen, about half of concocelis cells were alive.
日本水産増殖学会の論文