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<I>Objective</I>: To study the effect of Metalloendopeptidase-F (MEP-F), a major active ingredient of the anti-inflammatory drug Pronase, on HIV-1 in vitro.<BR><I>Materials and Methods</I>: HIV-1/LAI and AD8 stock viruses were treated with various concentrations of MEP-F and then incubated in PHA-activated normal human peripheral blood mononuclear cells (PBMC) for 5 days. PBMC or PHA-blast cells were treated with MEP-F either before or after infection with HIV-1. The cells were cultured for 5 days after infection. HIV-1 replication was evaluated by p24 antigen measurement using ELISA. The effect of MEP-F on cell surface antigens was analyzed by fluorescence-activated cell sorter (FACS).<BR><I>Results</I>: Treatment of HIV-1/LAI and AD8 with MEP-F resulted in a decreased viral infectivity. When PHA-blast cells infected with HIV/LAI were cultured in the presence of MEP-F, a dose-related inhibition of HIV-1p24 production was observed. Furthermore, when PBMC were treated with MEP-F prior to infection with HIV-1/LAI and then cultured in the medium with PHA added, the production of HIV-1 p24 antigen was significantly reduced. FACS analysis of cell-surface markers on MEP-F-treated PBMC revealed a remarkable decline of CD4 and CXCR4 levels expressed on CD3<SUP>+</SUP> T cells.<BR><I>Conclusion</I>: It is suggested that MEP-F is effective against HIV-1 infection by acting on the surface proteins of both the virus and the host cell.
- 日本エイズ学会の論文
日本エイズ学会 | 論文
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