Comparative mapping and structural analysis of a <I>FOX</I> gene cluster on bovine chromosome 18
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概要
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<I>FOX</I> genes encode a family of transcription factors which play important roles during early development and in regulation of metabolic homeostasis in mammals. Some members of the gene family, including <I>FOXC2, FOXF1</I>, and <I>FOXL1</I> genes comprise a gene cluster on particular regions of human and mouse chromosomes. In this paper, we report chromosomal localization and structure of bovine <I>FOXC2, FOXF1</I> and <I>FOXL1</I> gene cluster and comparative analysis of the gene cluster in cattle, human, and mouse, which were performed by a combination of <I>in silico</I> database searching and <I>in vitro</I> sequencing and mapping experiments. BLAST search of cattle database with cDNA sequences of human and mouse <I>FOXC2, FOXF1</I> and <I>FOXL1</I> genes identified several ESTs which represent cattle cDNAs of these genes. Radiation hybrid mapping of these cattle cDNAs revealed that the bovine <I>FOXC2, FOXL1</I>, and <I>FOXF1</I> genes are localized on the proximal region of bovine chromosome 18 and form a gene cluster. A comparative map of the region including the <I>FOX</I> gene cluster between cattle and human indicated that the localization of these genes on cattle chromosome is in concordant with those in the corresponding human chromosome. Then, we identified a contig of BAC draft sequences containing genomic sequences of these genes by MEGABLAST search of a cattle genome database using the cattle cDNA sequences. Complete genomic sequences of these genes were determined by cloning and sequencing of a part of genomic region in which the nucleotide sequences could not be obtained from the published BAC draft sequences. A comparison of the nucleotide sequences of the cattle, human, and mouse <I>FOX</I> gene clusters showed highly conserved structure of the cluster in mammalian species and identified some potential regulatory regions which are conserved in mammalian species.
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