Sequence analysis of functional Mhc class II β genes on quails of high- and low-IgG strains, developed by line breeding based on serum IgG concentration

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In order to clarify the immunological and genetic features of functional Mhc class IIβ genes in chicken and quails, we established a modified RT-PCR method, which classify to major or minor B-Lβ genes. Using this methodlogy, we classified quail Mhc class IIβ (CojaIIβ ) sequences to major or minor CojaIIβ, and found interesting features of functional MhcIIβ genes by detail comparisons between the quail and chicken, and the quail HTUA and LTUA lines. First, one major and one minor B-Lβ genes was identified in the chicken B21 haplotype. Four major and six minor CojaIIβ genes in HTUA line and two major and seven minor CojaIIβ genes in LTUA line were identified in quail two lines, respectively. Major genes were highly expressed in most of organs but minor genes were lowly expressed in a part of organs. Meanwhile, minor CojaIIβ genes weakly expressed only in some immune organs, and this phenomenon suggest that these genes specifically appears depending on the modality and maturity of lymphocytes.
日本動物遺伝育種学会の論文