Usefulness of DNA Fingerprinting for Swine

概要

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For obtaining clear DNA fingerprint and detecting variation with the bacteriophage M13 probe in swine, <I>Msp</I> I was suitable enzyme in the several restriction enzymes examined. Parentage test was carried out with fingerprints of Meishan Pig, Jinhua Pig and their F1 offspring. All bands of F1 were always originated from either parent. DNA fingerprinting analysis of their breeds (Meishan Pig, Junhua Pig and Landrace) and synthetic swine showed that their banding pattern is specific to each individual. Comparison with these banding patterns suggested the pattern is more similar within breeds than between breeds. Thus, DNA fingerprinting is useful not only for identifying the individual and parent-offspring but also for classifying the breeds of swine.
日本動物遺伝育種学会の論文