Thermoactive analyzer as a procedure to measure neutrophilic heat generation induced by various stimulators.

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Human neutrophils exist in blood and take part in defense mechanisms of the body.<BR>In this study the generation of heat produced by neutrophils was measured using thermoactive analyzer ESCO-3000. When neutrophils phagocytyzed zymosans, they generate heat in proportion to the amount of zymosan added. Cytochalasin B could reduced generation of heat from 17.6μW down to 2.8μW. Similar result was obtained when IgG-coated latex was used as an inducer. Phorbol myristate acetate (PMA) and Ca-ionophore A 23187 also could stimulate neutrophils to produce heat, but N-fomyl-L-methionyl-L-leucyl-L-phenylalnine (FMLP), and concanavalin A (Con A), failed to produce heat from neutrophils even when large amounts of FMLP and Con A were challenged. These phenomena suggest FMLP and Con A activate different metabolic pathways from zymosan, PMA or Ca-ionophore.<BR>These results, suggest that cellular membrane of granulocytes can recognize foreign substance electrically and physiologically, and after these foreign substances combine with the ligands of the membrane, structural changes of the membrane occur, and various substances such as Ca<SUP> _??_</SUP>, phospholipid metabolite and, cyclic nucleotides become transmitter, and produce heat inside neutrophils.
日本炎症・再生医学会の論文