Therapeutic effects of an antithrombin agent "Argatroban" on the experimental brain injury of rats by immunohistochemical study.

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Objects: To assess the effects of Argatroban which was developed as an antithrombin agent, we experimentally investigated the number of inflammatory cells and astrocytes infiltrated and/or appeared in injured lesion of rat brain.<BR>Methods: Gelatins soaked with Argatroban or saline were placed in the artificial cavities of brain defect for assessing the grade of infiltrating inflammatory cells using ordinary hematoxylin-eosin (HE) and immunohistochemical (IHC) stains. Polymorphonuclear leukocytes by HE stain and monocyte/macrophage positive cells, glial fibrillary acidic protein (GFAP) positive and vimentin positive astrocytes by IHC stain were compared between Argatroban and saline (control) treated group.<BR>Results: Argatroban suppressed accumulation of inflammatory cells, both polymorphonuclear leukocytes and monocyte/macrophage positive cells, and vimentin positive astrocytes along the brain defect edge, but had no effect on GFAP positive astrocytes along the brain defect edge.<BR>Conclusion: Argatroban may minimize the secondary brain damage through suppressing the infiltration of inflammatory cells and excessive gliosis comprised of vimentin positive astrocytes and have good effects on the neural regenaration.
日本炎症・再生医学会の論文