Movement of activated leukocyte and platelet in Behcet disease: Visual observation using artificial microchannels.:Visual observation using artificial microchannels

概要

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Interaction of both leukocytes and endothelial cells is important for inflammation. Its process develop in the venules of microcirculation system. Thus, we visually observed flow movement of leukocyte and platelet of Behçet disease patients using artificial microcirculation model. Venous blood of fourteen patients of Behçet disease and fifteen healthy controls were collected into syringe containing EDTA, respectively. Blood sample were whole blood diluted 4 times with plasma and PAF (10-6M) added to whole blood. Blood sample flow into modified cell-flow microchannels in a singlecrystal silicon substrate and the time for 100 μl of blood sample to pass through was measured. Deformity of leukocyte (might be rolling and adhesion) and platelet aggregation were visually observed in Behçet disease patient on TV monitor. And also, mean passage time of 100 μl sample prolonged in Behçet disease patient (147.92±25.74 sec.) compared with healty control (103.91±7.58 sec.) . This study suggested that leukocyte and platelet were already activated in patients with Behçet disease, and activated these cells resisted to flow through the microchannels and hence interfered with the flow of other blood components.
日本炎症・再生医学会の論文