Establishment of monoclonal antibody which blocks the adherence of activated-T lymphocytes to human gingival fibroblasts(HGF).

概要

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Molecular mechanism (s) involved in adhesive interactions between T lymphocytes and human gingival fibroblasts (HGF) were examined. Both human peripheral blood T lymphocytes and T cell leukemia line, Molt-4 demonstrated significant binding ability to HGF only when the cells were activated with phorbol 12-myristate 13-acetate (PMA) . In order to reveal the molecule (s) responsible for the adherence, a panel of monoclonal antibodies (mAbs) was prepared against PMA-activated Molt-4 and mAb, 4-145 was finally entablished on the basis of its ability to block the adherence of activated Molt -4 to HGF. Biochemical and functional analysis revealed that 4-145 recognizes an epitope on VLAβ1 chain. Furthermore, blocking experiments utilizing mAbs specific for VLAα chains demonstrated that VLA-4 plays an important role for mediating the adhesion of activated T lymphocytes to HGF.<BR>Results demonstrated in this study suggest that VLA integrins play critical roles not only in binding to activated endothelial cells in inflammatory sites but also in retention of activated T lymphocytes in inflamed periodontal lesions by mediating the adhesive interaction between T lymphocytes and HGF.
日本炎症・再生医学会の論文