Evaluation and investigation of active oxygens production in human monocyte using fluorescence probe.

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Active oxygens production in human monocyte was evaluated using two fluorescence probes, 2', 7'-dichlorofluorescin diacetate (DCFH-DA) and the anti-CD11c monoclonal antibody conjugated phycoerythrin (PE), on condition that the influence of neutrophil and plasma was removed. As results, in case of PMA stimulus, the active oxygens production of the inflammation and cancer disease has heightened significantly in comparison with the healthy controls. But, in case of phagocytic stimulus by opsonized zymosan, there was not a significant difference. This suggested that the decline of phagocytic function in monocyte of these diseases was reflected about the active oxygens production by phagocytic stimulus.<BR>We investigated about the influence of metabolites of arachidonic acid on the reaction system. As results, it was showed that there was not a influence of prostaglandin (PG), but it was not clear about a influence of leukotriene (LT) . Then, it was showed that the active oxygens production decreased remarkably by addition of plasma in the reaction system.
日本炎症・再生医学会の論文