Effect of high molecular weight sodium hyaluronate(SL-1010) on human neutrophil function.
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概要
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The effects of four different average molecular weights (MW) ; 0.47, 0.95, 1.52 and 2.0 × 10<SUP>6</SUP>, respectively of sodium hyaluronate (HA) designated those each as HA-47, HA-95, HA-152 and SL-1010, on the neutrophil functions including chemotaxis, adhesion to the plastic plate, phagocytosis and superoxide generation, and expression of CD44 antigen were studied.<BR>The inhibitory effect of HA on neutrophil chemotaxis was found to be dose (0.1-0.4 mg/ml) - and MW (0.47-2.0×10<SUP>6</SUP>) -dependent. Neutrophils were also observed to be inhibited to adhere to a plastic plate when the cells were treated with 10 μg/ml each type of HA. However, only SL-1010 inhibited the cell adhesion at the concentration of 1 μg/ml. Phagocytosis of FITC-latex particles was also found significantly inhibited by the HA-152 and SL-1010, but not by the HA-47 and the HA-95 at the concentration of 1 mg/ml. These inhibitory effects on the chemotaxis and phagocytosis observed in the presence of HA were similarly observed when neutrophils were pretreated with and washed out SL 1010.<BR>The generation of superoxide anions by stimulation with opsonized zymosan (OZ) was inhibited by the addition of HA, and this effect was also found to be dose- and MW-dependent.<BR>The effect of HA on neutrophil activating antigen, CD44 was analysed after the stimulation with OZ, and the results revealed that the CD44 expression on neutrophil was down-regulated by the stimulation. The down-regulation of CD44 was inhibited by HA in a MW-dependent manner.<BR>These results indicated that HA, especially the high MW SL-1010 might be a modulator against the activation of neutrophil to the inflammatory cascade.
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