Establishment of highly sensitive and wide-ranged immunoassay for TNF.ALPHA. by DELFIA.
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概要
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Non-isotopic immunoassay for the human tumor necrosis factor α (TNFα) was established by employing the dissociated enhanced lanthanide fluoroimmunoassay (DELFIA) system, based on the timeresolved fluoroimmunoassay (TR-FIA) technique utilizing the europium-labeled antibody. In comparison with enzyme-linked immunosorbent assay and bioassay, the sensitivity and range of measurement could be significantly increased by applying the DELFIA system to TNF<SUB>α</SUB>.<BR>Biological samples, including serum, may affect some of the immunoassays. We overcome the serum influence to the measurement of TNF<SUB>α</SUB> by DELFIA, Percent recovery of additional TNF<SUB>α</SUB> in each diluted serum was determined, then the sliding standard curve of each serum sample was mathematically calculated from the recovery. Finally corrected TNF<SUB>α</SUB> amount was detected by using the sliding standard curve. We suggest that more accurate, sensitive and wide ranged TNF<SUB>α</SUB> immunoassay has been established by DELFIA with some revising calculation.
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