Intraperitoneal amyloid formation by amyloid enhancing factor-rich macrophages in ascitic fluid.

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Although peritoneal resident cells from amyloidotic mice (amyloidotic peritoneal cells) are capable of processing serum amyloid A (SAA) to amyloid fibrils, the original residence peritoneum is a rare place of amyloid deposition. This is considered to occur due to deficiency of blood supply in the peritoneum. To increase blood supply in the peritoneum, ascitic fluid which contained about the same proteins as those in the serum was induced in mice.<BR>Amyloidotic peritoneal cells were packed in a microchamber and cultured in ascites with additional inflammatory stimuli which increased the SAA concentration of ascites to about 1% of the highest serum level.<BR>On the 7 th day, Congo red positive structures which showed green birefringence under polarized light were found inside and occasionally outside the chamber. By anti-AA or-SAA immunostaining, amyloid deposits as well as cell surface of adjucent macrophages were positively stained. Immunologic depletion of T and B lymphocytes from amyloidotic peritoneal cells did not adversely effect the amyloid formation in microchambers.<BR>These results suggest that both ascitic fluid containing sufficient amount of SAA and peritoneal macrophages with high AEF activity are indispensable agents for AA amyloid fibrillogenesis in the peritoneum.
日本炎症・再生医学会の論文