Isolation and characterization of cDNA clones for neutrophil cationic peptides.

概要

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cDNA clones encoding antimicrobial guinea pig neutrophil cationic peptides GNCP-1 and GNCP-2 were isolated from a bone marrow cell cDNA library. Analysis of these clones indicated that both GNCPs were produced as precursor proteins com-prising 93 amino acid residues, which were composed of signal sequences (N-terminal 19 residues), pro-peptide sequences (43 residues) and mature GNCP sequences (31 residues) . The deduced amino acid sequences showed that there were only two amino acid differences between GNCP-1 and GNCP-2, one in the pro-peptide region and another in the mature peptide region. Interestingly, Northern blot analysis and transcription run-off assay revealed that the expression of GNCP mRNA and the transcription of GNCP gene was observed in bone marrow cells but not in mature neutrophils.<BR>These observations suggest that GNCP is synthe-sized by maturing neutrophils in the bone marrow.
日本炎症・再生医学会の論文