Dose dependent decrease in LTB4 formation of rat leukocytes by the administration of EPA ethyl ester.

概要

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To clarify the anti-inflammatory effect of eicosa-pentaenoic acid (EPA), we investigated the effect of EPA administration on A23187-stimulated LTB4 formation by rat leukocytes. LTB4 is known to be an important mediator of inflammation and the decreased formation of LTB4 is thought to be one of the important mechanism of anti-inflammatory effect of EPA. EPA ethyl ester (EPA-E) administration (100 mg, 300 mg and 1, 000 mg/kg/day) for 4 weeks dose dependently suppressed the formation of LTB4 and increase in the formation of LTB5 with the increase in EPA content in rat leukocytes. The content of docosapentaenoic acid (DPA) also dose-dependently increased and the content of arachidonic acid (AA) and docosahexaenoic acid (DHA) were decreased by the administration of 300 mg and 1000 mg of EPA-E daily. The formation of LTB4 in stimulated leukocytes and the content of AA are positively correlated. FMLP-induced chemotactic activity of leukocytes were suppressed with the EPA-E treatment but FMLP stimulated β-glucuronidase release was not affected by EPA administration. In conclusion, highly purified EPA-E administration reduced the formation of proinflammatory LTB4, increased the LTB5, which would competitively inhibit the action of LTB4 and suppressed the chemotactic activity of leukocytes, these might result in the suppression of inflammatory response.
日本炎症・再生医学会の論文