The role of the protease-protease-inhibitor complexes

概要

論文の詳細を見る
It is well known that at the site of inflammation neutrophils release proteolytic enzymes which cause tissue damage. These proteases combine with protease inhibitors such as α1-antitrypsin and α2-macroglobulins and lose their biological activity. We examined the fate of these proteaseprotease inhibitor complexes, α1-antitrypsin was used as a protease inhibitor and purified by passing through the blue sepharose and sephadex columns. Trypsin was used as a proteolytic enzyme and mixed with purified α1-antitrypsin at 37°C to make a proteaseprotease inhibitor complex. This complex was separated from native α1-antitrypsin by isoelectric focusing and then incubated with normal human neutrophils. Significant lysosomal enzyme release was seen when neutrophils were incubated with this protease-protease inhibitor complex, but such enzyme release was not observed when neutrophils were incubated with native α1-antitrypsin or trypsin. The amount of lysosomal enzyme release was proportional to the amount of complex added. From these result we presume that this complex can promote the further protease release from the cells and worsen the in flammation.
日本炎症・再生医学会の論文