Involvement of plasma kallikrein-kinin system in experimental rat pleurisy induced by intra-pleural injection of carrageenin

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Experimental pleurisy in rats was induced by intra-pleural injection of 0.1 ml 2%-λ-carrageenin, as previously reported (1, 2) . The accumulation of pleural fluid continued until the peak volume of 4 ml at 19 hrs after carrageenin injection, involvemen of plasma kallikrein-kinin system in the early phase of the pleurisy was proved with the following series of experiments. (1) The levels of prekallikrein, HMW-and LMW-kininogens in plasma and pleural fluid were measured at 1, 3 and 5 hrs after carrageenin injection. The level of HMW-kininogen in pleural fluid was extremely low, whereas LMW-kininogen level was not reduced. The level of prekallikrein was also low in the pleural fluid, about 1/10 of that in plasma. As the plasma levels of these components in pleurisy rats were not significantly different from those in control rats, the above results suggest that plasma kallikrein-kinin system could be activated at the inflammatory site, but not in circulating blood. (2) Pretreatment with 10 mg/kg intraperitoneal captopril enhanced significantly the accumution of pleural fluid as well as dye exudation. (3) Pretretament with bromelain, 10 mg/kg intravenously, which was shown to exhaust HMW-kininogen and prekallikrein, suppressed the accumulation of pleural fluid and dye exudation, significantly at 1, 3 and hrs after crageenin.
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