SA 96: An immunopharmacological profile in vitro

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We have screened from various thiol compounds SA 96 (2-mercapto-2-methylpropanoyl-L-cysteine) as a stimulant of LPSinduced proliferative response of murine lymphocytes, and the immunopharmacological profile of this compound was studied in vitro. SA 96 caused 3H-thymidine (3H-TdR) uptake only in the presence of fetal calf serum (FCS) . The effects of SA 96 on the proliferative response to mitogens were biphasic. The uptake of 3H-TdR induced by Con A or LPS was suppressed in a dose-dependent manner below 10-4 M, but was enhanced at 10-3 M in the case of LPS. The response to Con A was recovered from the suppression at 10-3 M but did not exceed the control level. Some experiments suggested that cysteine moiety might importantly contribute to the proliferative response caused by SA 96. SA 96 slightly enhanced the PFC response to SRBC in vitro, but markedly inhibited the elevated response in the presence of suboptimal concentrations of mercaptoethanol (2-ME) . On the other hand, PFC response to DNP-Ficoll (a thymusindependent antigen) was augmented by SA 96 even at its non-mitogenic doses irrespecitve of the presence of 2-ME. These findings suggest that SA 96, a thiol compound may possibly be useful as an immuno-stimulant like levamisole.
日本炎症・再生医学会の論文