STRUCTURAL PROTEINS OF C-TYPE RNA LEUKEMIA VIRUSES AND AUTOIMMUNE DISEASES OF NEW ZEALAND MICE:BIOCHEMICAL ANALYSIS OF MuLV STRUCTURAL PROTEINS IN THE SERUM AND MuLV CELL SURFACE ANTIGENS DETECTABLE BY ENDOGENOUS ANTIBODY
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Gp70-related molecules in the serum were heavily deposited in the lupus kidney and vascular lesions as an immune complex in New Zealand mice. They were characterized biochemicallyby using affinity chromatography and SDS-PAGE analysis. Isolated gp70-related molecules in the serum consisted of three glycoprotein fragments with an approximate molecular weight of 53, 000, 45, 000 and 32, 000 daltons, respectively. In contrast, gp70 isolated from the cell surface affected with mouse leukemia, E_??_G2, was shown to be a single glycoprotein molecule with a molecular weight of approximately 70, 000 daltons.The major gp70 activity in the serum fractionated by Sephadex gel filtration was found to be in a range of molecular weight from 120, 000 to 140, 000. The minor gp70 activity was found to be of the same size as serum albumin. All the results obtained suggest that the three glycoprotein fragments of serum gp70 described in this study might have resulted either from specific transcriptional, translational, or post-translational process, or from nonspecific proteolysis at susceptible sites. It is possible that a gp70-related molecule in the serum may be a specific envelope-related glycoprotein which consists of three glycoprotein fragments with a molecular weight of 53, 000, 45, 000 and 32, 000 daltons, respectively. The alternative is that serum gp70 may be either associated with host cellular components or present as a dimer.Pooled B/WF1 mouse sera from 2 age groups were fractionated by Sephadex gel filtration. The major gp70 activity of 2-month-old B/WF1 serum was found in fractions with a molecular weight in a range from 120, 000 to 140, 000 daltons. In contrast, the gp70 activity of 9-month-old B/WF1 serum eluted for the most part at the void volume. The 125I-labeled gp70-anti-gp70 immune complex prepared in vitro eluted also at the void volume. It was suggested that gp70-related molecules in the serum of aged B/WF1 mice were present largely as immune complexes.In attempts to identify directly the molecule(s) bearing the antigenic determinants for endogenous antibody to MuLV, or Gross natural antibody (GNA), 125I-E_??_G2 MuLV proteins were subjected to immunoprecipitation with GNA-positive mouse sera. SDS-PAGE analysis of the resulting precipitates revealed that the major molecule bearing antigenic determinants for GNA is p (85), the precursor polyprotein of the MuLV core structural proteins, p 30, p 15, and p 10.
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