EFFECT OF ULTRAVIOLET LIGHT ON THE MULTIPLICATION OF RACTEIOPHAGE:(1) STUDIES ON THE BACTERIOPHAGE REPRODUCTION OF ULTAVIOET LIGHT IRRADIATED E. COLI INFECTED WITH T2

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Bacteriophage T2 can produce progeny phage in cells of E. coli which had been inactivated by ultraviolet irradiation. In this case, however, the latent period increases and the burst size decreases as compared to the infection in normal cells. The degrees of elongation in latent period and decrease in burst size depend on dose of ultraviolet irradiation. This phenomenon was examined with various doses of UV using both Iris-glucose and peptone-glucose media.When irradiation was performed at 30cm from the source of ultraviolet light (15w), the increase in latent period was about 20 minutes in tris-glucose medium and about 30 minutes in peptoneglucose medium. The burst size decreased by one third.When bacterial cells grown in tris-glucose medium were used, a stage, which might be called an incomplete infection, was observed during infection period. This phenomenon appears to enhance the increase of latent period with cells grown in this medium over that with cells grown in peptoneglucose medium. The precise reason for this is as yet unknown.
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