IMMUNITY TO INFECTIOUS ECTROMELIA IN MICE RELATION BETWEEN LOCAL LESION AT THE SITE OF INOCULATION AND THE SYSTEMIC INFECTION
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Some problems in the immunity to infectious ectromelia were discussed in a previous report. In the present paper, the immunity was studied with special reference to relationships between the immunity to the systemic disease and that to the local lesion at the inoculated site.In neutralization test, when a mixture of virus and homologous immune serum was injected intraperitoneally the neutralizing effect was measurably shown, while when injected plantarly local lesions developed with similar intensity as in controls in which only virus was inoculated. Then, virus, homologous immune serum, and the peritoneal fluid of mice were mixed and injected after incubated at 37°C for 2 hours and subsequently in an ice-box overnight. As shown in Fig. 1, local lesions caused by plantar inoculation were again similar as in the controls.In order to know the virus-absorbing ability of immune serum in vitro, homologous immune serum was mixed with virus, incubated at 37°C for 2 hours and in an ice-box overnight, and then heated at 56°C for 30 minutes. The supernatant after a centrifugation in 12, 000 r. p. m. for 60 minutes was used for neutralization and complement fixation test. By this method no virus-absorption by immune serum was confirmed.When mice surviving infectious ectromelia were reinoculated, they developed neither local lesions nor generalized diseases. In mice treated with U. V.-irradiated virus, local lesions by plantar injection were developed in a similar intensity as in controls, but no generalized diseases were observed. This result was confirmed by the titration of virus in the blood, liver and pad of the mice after infection.Moreover, when mice were daily injected with immune serum in early stages after intraperitoneal infection with virus no systemic symptoms could be found, while local lesions were caused by plantar infection regardless of the serum injections.From the results mentioned above it seems to be safely concluded that the systemic infection of mice with ectromelia virus could be protected by serum antibodies, whereas the protection of local disease at the pad could be obtained only after infection with live homologous virus and has no relation to the existence of serum antibodies. Therefore, the pathogenesis and immunity of these two types of diseases in infectious ectromelia should be separately considered.
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日本ウイルス学会 | 論文
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