STUDIES ON THE GROWTH CYCLE OF ECTROMELIA VIRUS PROPAGATED IN EHRLICH ASCITES TUMOR CELLS

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A new virus-host system or ectromelia virus-Ehrlich ascites tumor cell system has been introduced. With this system the growth curve of this virus was showed in vivo and in vitro, and at the same time parallel morphological changes of these cells were demonstrated; adsorption, immigration, eclipse, latent period, multiplication, and liberation were suggested.In vivo type B of the inclusion bodies (matrices) appeared at first six hours after the infection, and type A of these (Marchal bodies) fourteen hours. Then they increased more and more in number and in size. Eighteen hours after the infection oncolyses came in view. About three days later, the majority of the cancer cells disappeared as the result of oncolyses. Mitoses have been decreased or disappeared. The first step of growth cycle continued for ca, eighteen hours, and each following step about twelve hours.Also in vitro the inclusion bodies and mitoses have been demonstrated. One-step growth cycle has been prolonged for two or three days. Type B of the inclusion bodies appeared on the second day of the incubation, they increased on the third day, and type A of the inclusion bodies appeared on the fourth day.Quantitative studies on the interaction between the virus and the tumor cells have been done.Type A and type B of the inclusion bodies had been described in the previous report of the author.
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