STUDIES ON THE MORBID ANATOMICAL CHANGES OF VACCINATED RABBITS

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Histological changes of the skin inoculated with vaccine virus by means of the sand paper method are observed fromimmune biological point of view. (See P. 108)1) After 5 hours of this vaccination, epidermis cell changes to necrosis and the neutrophilic infiltration appears in demarcation zone. When these tissues discharged after 5 days, there prepared already regenerated epidermis beneath the scab. However, these changes are seen in the contrast experiment by simple sand paper rabbing or electric burning. So the charactericity of the vaccinated epidermis should be represented by the regenerated epidermis in the following stage.2) The inclusion body appears in the cell body of the regenerated hypertrophic epidermis and the follicule epithelium after 2 days and till 7th day. After 4 days a part of the hypertrophic epidermis is liquidated calling the infiltration of the neutrophilic cell and postulating the locus. Following this reaction the monocytic infiltration appears in the papilla, growing gradually to the depth of the corium along the hair follicule and reaching to the reticular layer.3) After 8 days the hemorrhagic inflammation appears in the monocyte infiltrated corium which makes this leisions necrotic and causes to fall off. After this time neither the inclusion bodies nor the monocytic infiltration were to be found. These findings show that there is a close correlation between the existence of the inclusion body and the monocytic reaction in the epidermis. It can be said that the tissue reaction against the vaccinia virus is the monocytic nature.4) The colour of the neutral-red vacuoles of the histiocyte in the reticular layer was observed supravitall, which changed to brownish during the period of 3 to 17 days after the vaccination in parallel to the alkalosis which occured the inoculated tissue.5) The plasma cells began to grow close to the addventitia cells in the subcutaneous tissue 4 days after the inoculation. After 7 days they infiltrate in cluster and after 8 days explosively around the area of the hemorrhagic infiltration. The proliferation of the plasma cell occurs on the period of alkalosis of the inoculated locus as well as the bone marrow successive to the leucocyte infiltration and alkalosis of the tissues.6) 20 to 25 days after the primary vaccination the reinfection experiment was applied to the abdominal side of the animals (for the observation of the Pirquet's immediate reaction). The erythema and the swelling of the inoculated locus reached to its maximum after 24 hours transitorily without any following pustulation.7) The neutrophilic reaction on the vaccinated locus at the beginning of the second inoculation appeared more intense than that of the primary, somewhat like the mode of Arthus Phenomenon.8) As the destruction of the virus was performed by this Phenomenon so neither the inclusion body nor the monocytic infiltration was to be seen in this reinoculation. However, the call of the plasma cell reaction is rather quickened.9) pH of the locus is alkalified in parallel to its erythema and then returned to the normal state in a short time. However, the brown colour of histiocyte-vacuoles is still remained after the recovery of the tissue pH, which means brown colour of histiocyte-vacuoles is not influenced simply by the tissue pH.
日本ウイルス学会の論文

STUDIES ON THE MORBID ANATOMICAL CHANGES OF VACCINATED RABBITS

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概要

日本ウイルス学会 | 論文

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