STUDIES ON THE TYPES OF INACTIVATION OF PURIFIED TOBACCO MOSAIC VIRUS (TMV):I. Experiments with temperature, sonic waves, formalin, extract from Phytolacca americana and "bukulin" extract from Pachyma hoelen
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Morphology, pathogenecity and serological activity of purified TMV have been studied under treatments with temperature, sonic waves, formalin, extract from the leaves of Phytolacca americana L. and "bukulin" extracted from Pachyma hoelen Rumph.1. In experiments with varying temperature no change was observed at 75°C. At, 80°C or a higher temperature the number of about 280mμ long rods was considerably reduced and the number of short particles accordingly increased. The number of the latter became very large at a temperature of 90°C or more. With the increase of the number of small particles the pathogenecity was decreasing, while, on the other hand, the serological activity was growing.2. In experiments with sonic waves the longer the treatment lasted the more reduced became the length of the rods; they did not reach the size mentioned above. The pathogenecity was reduced and the serological activity increased.3. In experiments with temperature and sonic waves the length of the shortest particles was about 25mμ or still less, and their number was increasing in advanced treatment stages.4. In experiments with temperature the short particles, enlarged in size, showed more or less irregular hexagonal contours. Sporadically they were seen standing upright; the same phenomenon could be observed under treatment with sonic waves.5. In experiments with sonic waves though the rods underwent segmentation, often the particles did not disperse. It is supposed that they were kept together by some substance. (See Fig. 1-Fig. 12, page43-48.)
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