Isolation of Shiga Toxin-Producing E. coli in Slaughterhouses.
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概要
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For isolation of Shiga toxin-producing <I>Escherichia coli</I> (STEC) from dressed cattle carcass, a combination of 25 mg/<I>l</I> novobiocin added m-EC medium before sterilization, immuno-magnetic separation technique and cefixime-tellurite sorbitol MacConkey agar, was found to be most useful and screening of secondary enrichment cultures after the immunomagnetic separation using an immunoprecipitate assay kit was recommendable as a rapid method. Using these methods, 13 STEC-O157 strains were detected from the cattle feces and equipments, from July 1996 to September 1997 and all of them were found to have the eaeA gene where as only one of 32 STEC isolates without O157 possessed the gene.
- 公益社団法人 日本獣医師会の論文
公益社団法人 日本獣医師会 | 論文
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