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A new detection method of bovine <I>Sarcocystis</I> cysts (sarcocysts) was devised. In it, a small piece (about 2×5×0.5cm in size) of cardiac muscle was cut out at rightangles to the fibers and examined by a binocular dissecting microscope with illumination from above. Sarcocysts could be observed as minute whitish bodies. The new method was superior to the conventional one in the following points: Fresh sarcocysts could easily be removed from muscle with a dissecting needle. They could be used for morphological examinationin order to identify the species and for experimental infection of final hosts, such as dogs.<BR>The new method was applied to 104 healthy cattle slaughtered. It revealed that the incidence rate of sarcocysts increased with the advance in age, that it was 100% among cattle more than 5 years old, and that the larger the cyst, the larger the number of bradyzoites contained.
- 公益社団法人 日本獣医師会の論文
公益社団法人 日本獣医師会 | 論文
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