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Antigen used was physiological salin extracts from adult flukes of <I>Fasciola</I> sp. When it was allowedto react with sera from 209 cattle, two or three precipitating lines appeared. Specificity ofthese reactions was demonstrated by the immunodiffusion reaction (ID) and a combination of IDand absorption test.<BR>By using 0.8% agar in 0.05M veronal-HCL buffer, pH 8.6, counterelectrophoresis (CEP) wascarried out to detect precipitating antibody in the sera of 16 cattle in the pasture. <I>Fasciola</I> eggs werefound 2 months after the appearance of the antibody in the serum. After that, no antibody could befound CEP was more capable of detecting early infection than ID.
- 公益社団法人 日本獣医師会の論文
公益社団法人 日本獣医師会 | 論文
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