DNA Fingerprinting of Escherichia coli O157 by Infrequent-Restriction-Site PCR.
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概要
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Pulsed-field gel electrophoresis (PFGE) has been used as a reliable epidemiological tool to identify the possible source and route of infection by Shiga-toxin producing <I>Escherichia coli</I> (STEC) O157. However, it has been found that some strains could not be subjected to the analysis because their DNAs are easily degraded. To produce strain-specific profiles of such strains, we used a method of amplifying DNA sequences flanking infrequent restriction sites (infrequent-restriction-site PCR; IRS-PCR) . Fifty-two strains of <I>E. coli</I> O157 were typed into 12 and 14 types by IRS-PCR patterns from DNAs digested with <I>XbaI</I> and <I>HhaI</I> and those with <I>Bln</I>I and <I>Hha</I>I, respectively. These data suggest that selection of an appropriate set of restriction enzymes will provides more discrimination patterns. In 18 of the 52 strains, that gave individual PFGE patterns, 11 IRS-PCR patterns were shown. Remaining 34 strains that were untypeable by PFGE were classified into 3 IRS-PCR types, indicating that some of those strains were possibly related.
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