Morphological Studies on Junctional Complexes in Compacted Mouse Embryos Developed in a Tunicamycin-Containing Medium

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Morphological states of junctional complexes were compared between two groups of compacted mouse embryos: one developed in a tunicamycin-containingmedium (TCM) and the other in at unicamycin-free medium (TFM). Both groups of compacted embryos had gap junctions between all pairs of round blastomeres, and between a round blastomere and a flattened one, while had zonula occludens, predesmosomes and gap junctions between all pairs of flattened blastomeres, but in embryos developed in a TCM, the junctional areas of the zonula occludens and of each predesmosome were smaller, and the number of the latter was fewer. While actin fluorescence showed no difference as to location and strength between the two groups, cytokeratin fluorescence did: it was filamentous and stronger in the cytoplasm of blastomeres in the embryos developed in a TFM, whereas it was granular and weaker when developed in a TCM. Decompacted embryos cultured in a TCM, meanwhile, showed no junctional complexes nor actin fluorescence, though they showed very weak cytokeratin fluorescence.
日本哺乳動物卵子学会の論文