In vitro exposure of bovine immature oocytes to K99+Escherichia coli and elimination of exposed bacteria by washing
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概要
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Immature bovine oocytes were exposed in vitro to K99<SUP>+</SUP>Escherichia coli (K99 E. coli) to determine if K99 E. coli would adhere to the cumulus cells-oocyte complexes or adhere to the cumulus cells-free oocytes. And standard procedures for washing and treating embryos were evaluated to determine their effectiveness for removing K99 E. coli. K99 E. coli was isolated from all groups of cumuluscells-oocyte complexes and 2 of 3 groups of cumulus cells-free oocytes after in vitro exposure and subsequent washing. According to increase in the number of repeated washing, a total number of K99 E. coli isolated from washing medium declined. However after the eighth time of washing, the number of K99 E. coli isolated from washing medium did not decline in the groups of cumulus cells-oocytes complexes. These results indicated that standard procedures for washing were not effective for removing K99 E. coli adhered to immature oocytes and were not able to remove K99 E. coli adhered to the cumulus cells-oocyte complexes.
- 日本哺乳動物卵子学会の論文
日本哺乳動物卵子学会 | 論文
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