Effects of Calf Serum and Bovine Serum Albumin on the Compaction of the Early Rat Embryos
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Effects of calf serum (CS) and bovine serum albumin (BSA) on the compaction of 8-cell rat embryos were investigated.Brief culture of pre-compacted 8-cell rat embryos with a medium containing CS led to 100% compaction of the embryos.In contrast, BSA-treated and untreated (control) embryos showed no signs of cell flattening (compaction) even after 12h of culture.Approximately 30% of the CS-treated embryos developed into the blastocysts following 18h of incubation, and around 80% resulted in blastocyst stage after 24h of culture.These results suggest that the development of rat 8-cell embryos is probably influenced by CS which may have some factor (s) responsible for the stimulation of compaction.
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