Distribution of the number of penetrating sperm in zona-free mouse eggs fertilized in vitro
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概要
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Mouse eggs, freed from cumulus cells and zona pellucida, were inseminated at various sperm concentrations in vitro. The fertilization rates of these eggs were in direct proportion to the logarithm of sperm concentration. Sperm concentration for 50% fertilization rate in the zona-free eggs was about 1/25 of that for cumulus-free eggs. Frequency of the number of penetrating sperm in zona-free eggs closely fitted Poisson distribution at low sperm concentration (≤0.9 calls/μl) suggesting that the fertilization was dependent on the random collision of an egg and a fertile spermatozoon. At higher sperm concentrations, however, incidence of monospermic fertilization was significantly higher than that expected from Poisson distribution, showing the vitelline block in these zona-free eggs. When zona-free eggs were coincubated with sperm for restricted periods, sperm attachment started in almost all eggs with 30 sec and eggs with binding sperm reached 87%at 3 min. But these binding sperm were not able to penetrate into cytoplasm immediately and sperm exposure time for more than 30 min was requred to obtain high (>90%) fertilization rate. Increase in total sperm number in the fertilization medium resulted in a significant increase in the fertilization rate at a fix sperm concentration (10 sperms/μl). These result indicate that in vitro frtilization of zona-free mouse eggs is dependent on sperm: egg ratio in the fertilization medium.
- 日本哺乳動物卵子学会の論文
日本哺乳動物卵子学会 | 論文
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