An attempt to culture chimeric mouse embryos in a Brinster's Medium and Dulbecco's Modified Eagle Medium.

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Development of chimeric mouse embryos cultured in a Brinster's medium and a Dulbecco's modified Eagle medium (DMEM) was examined in an attempt to produce chimeric mouse using 8-16-cell stage aggregated embryos. Growth of chimeric mouse embryos cultured in a Brinster's medium or DMEM in vitro was slower than that of normal embryos in vivo. Both Brinster's Medium and DMEM were slightly modified by addition of glucose (0.2% concentration). When modified Brinster's medium and modified DMEM were used to culture aggregated chimeric embryos, growth of aggregated chimeric embryos were faster than aggregated chimeric embryos cultured in several basic media that containing 0.1% glucose. Furthermore, modified Brinster's medium and modified DMEM were incubated with ovaries for 2 hour in advance. When modified and conditioned Brinster's medium and modified and conditioned DMEM were used to culture aggregated chimeric embryos, growth of aggregated chimeric embryos were faster than aggregated chimeric embryos cultured in several modified and not conditioned media that containing 0.2% glucose. Transfer of aggregated chimeric embryos cultured in modified and conditioned Brinster's medium to recipients resulted in live pups.
日本哺乳動物卵子学会の論文