In vitro maturation and in vitro fertilization of eggs. recovered from ovaries of cynomolgus monkeys (<I>Macaca fascicularis</I>) at necropsy in a indoor breeding colony
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概要
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Cynomolgus monkey oocytes were collected from the ovarian follicles of females that died from accidents, diseases of respiratory and digestive organs, or sacrificed for the other experiment. Immature oocytes were collected from 10 females aged 1 to 18 years except 1 female aged 19 days and they were cultured for 24 h in TCM 199 medium supplemented with 10 % FCS and 10 IU/ml PMSG. The 11.9 % oocytes extruded the first polar body and some of them were further used for in vitro fertilization. In two cases, we could observe the presence of male and female pronudei and the second polar body. The ovaries were histlogically different with regard to the number of primordial follicles and the vesicular follicles between sexually immature and mature females. This study suggests that the ovarian oocytes collected from dead animals are useful for the embryological studies in nonhuman primates, and this procedure is applicable to gene banking for individuals died accidentally.
- 日本哺乳動物卵子学会の論文
日本哺乳動物卵子学会 | 論文
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